diff --git a/Marlin/Configuration_adv.h b/Marlin/Configuration_adv.h
index 40b59587b6..b885ba5bc3 100644
--- a/Marlin/Configuration_adv.h
+++ b/Marlin/Configuration_adv.h
@@ -1346,7 +1346,9 @@
 #if ENABLED(ENCODER_RATE_MULTIPLIER)
   #define ENCODER_5X_STEPS_PER_SEC    30
   #define ENCODER_10X_STEPS_PER_SEC   80  // (steps/s) Encoder rate for 10x speed
-  #define ENCODER_100X_STEPS_PER_SEC  130  // (steps/s) Encoder rate for 100x speed
+  #if DISABLED(DWIN_MARLINUI_PORTRAIT)
+    #define ENCODER_100X_STEPS_PER_SEC  130  // (steps/s) Encoder rate for 100x speed
+  #endif
 #endif
 
 // Play a beep when the feedrate is changed from the Status Screen
diff --git a/Marlin/src/lcd/e3v2/common/encoder.cpp b/Marlin/src/lcd/e3v2/common/encoder.cpp
index edfaf60aae..dcd1af2bb6 100644
--- a/Marlin/src/lcd/e3v2/common/encoder.cpp
+++ b/Marlin/src/lcd/e3v2/common/encoder.cpp
@@ -139,8 +139,10 @@ EncoderState Encoder_ReceiveAnalyze() {
           // Note that the rate is always calculated between two passes through the
           // loop and that the abs of the temp_diff value is tracked.
           const float encoderStepRate = encoderMovementSteps / float(ms - EncoderRate.lastEncoderTime) * 1000;
-               if (encoderStepRate >= ENCODER_100X_STEPS_PER_SEC) encoderMultiplier = 100;
-          else if (encoderStepRate >= ENCODER_10X_STEPS_PER_SEC)  encoderMultiplier = 10;
+          #if defined(ENCODER_100X_STEPS_PER_SEC)
+            if (encoderStepRate >= ENCODER_100X_STEPS_PER_SEC) encoderMultiplier = 100;
+          #endif
+          if (encoderStepRate >= ENCODER_10X_STEPS_PER_SEC)  encoderMultiplier = 10;
           #if ENCODER_5X_STEPS_PER_SEC
             else if (encoderStepRate >= ENCODER_5X_STEPS_PER_SEC) encoderMultiplier = 5;
           #endif
diff --git a/Marlin/src/lcd/marlinui.cpp b/Marlin/src/lcd/marlinui.cpp
index 8bf0e71d3c..353c593ccf 100644
--- a/Marlin/src/lcd/marlinui.cpp
+++ b/Marlin/src/lcd/marlinui.cpp
@@ -1026,8 +1026,10 @@ void MarlinUI::init() {
                   // loop and that the abs of the encoderDiff value is tracked.
                   const float encoderStepRate = encoderMovementSteps / float(ms - lastEncoderMovementMillis) * 1000;
 
-                  if (encoderStepRate >= ENCODER_100X_STEPS_PER_SEC)     encoderMultiplier = 100;
-                  else if (encoderStepRate >= ENCODER_10X_STEPS_PER_SEC) encoderMultiplier = 10;
+                  #if defined(ENCODER_100X_STEPS_PER_SEC)
+                    if (encoderStepRate >= ENCODER_100X_STEPS_PER_SEC)     encoderMultiplier = 100;
+                  #endif
+                  if (encoderStepRate >= ENCODER_10X_STEPS_PER_SEC) encoderMultiplier = 10;
 
                   // Enable to output the encoder steps per second value
                   //#define ENCODER_RATE_MULTIPLIER_DEBUG